Review



ca 2+ detection kit  (Beyotime)


Bioz Verified Symbol Beyotime is a verified supplier
Bioz Manufacturer Symbol Beyotime manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Beyotime ca 2+ detection kit
    Ca 2+ Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/ca2++kit/pmc11937690-75-8-12
    Average 90 stars, based on 1 article reviews
    ca 2+ detection kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    MTT Assay:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Imaging:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    In Vitro:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Viability Assay:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Fluorescence:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Flow Cytometry:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Expressing:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Infusion Solution:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Saline:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Colorimetric Assay:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Activity Assay:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Membrane:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Migration:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Cell Culture:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Immunofluorescence:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Staining:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures
    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Article Title: A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection
    Article Snippet: Ca 2+ concentration detection kit, Calcein AM/PI kit, MTT, H&E kit, and Masson kit were purchased from Beyotime (Shanghai, China).

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units
    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Article Title: Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation
    Article Snippet: The Ca 2+ detection kit Fluo 3‐Am (S1056) and 4′6‐diamidino‐2‐phenylindole (DAPI) (C1002) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China).



    Similar Products

    86
    Nanjing Jiancheng Bioengineering Research Institute Co Ltd ultramicro ca 2 atpase detection kits
    Transcriptomic results of PRAC-E ameliorating HA. ( A ) Venn diagram of the DEGs from PRAC-E vs. LYSO-6 and LYSO-6 vs. Normal. ( B ) LYSO-6 vs. Normal DEGs volcano plot. ( C ) PRAC-E vs. LYSO-6 DEGs volcano plot. ( D ) Functional enrichment analysis of downregulated DEGs in LYSO-6 vs. Normal. ( E ) Function enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( F ) Pathway enrichment analysis of the downregulated DEGs in LYSO-6 vs. Normal. ( G ) Pathway enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( H ) Ca 2+ -ATPase activity in liver tissue ( n = 6). Data are presented as the mean ± standard deviation (SD) ( n = 6). ** p < 0.01, *** p < 0.001 vs. LYSO-6.
    Ultramicro Ca 2 Atpase Detection Kits, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/assay+atpase+kit/pmc13026298-151-4-13
    Average 86 stars, based on 1 article reviews
    ultramicro ca 2 atpase detection kits - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Shanghai Haohai Biological Technology Co intracellular free ca 2+ detection kit
    The concentration of intracellular free Ca 2+ of cardiomyocytes in vivo
    Intracellular Free Ca 2+ Detection Kit, supplied by Shanghai Haohai Biological Technology Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/intracellular+free+ca+2++detection+kit/pmc12146963-68-14-27
    Average 90 stars, based on 1 article reviews
    intracellular free ca 2+ detection kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime ca 2+ detection kit
    The concentration of intracellular free Ca 2+ of cardiomyocytes in vivo
    Ca 2+ Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/ca2++kit/pmc11937690-75-8-12
    Average 90 stars, based on 1 article reviews
    ca 2+ detection kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime ca 2+ concentration detection kit
    The concentration of intracellular free Ca 2+ of cardiomyocytes in vivo
    Ca 2+ Concentration Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/calcein+pi+cell+viability+cytotoxicity+assay+kit/pmc11948004-143-0-17
    Average 90 stars, based on 1 article reviews
    ca 2+ concentration detection kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime fluo-4 ca 2+ detection kit
    In vitro effects of the combination hypothermia and MgSO 4 on neurons. (A) The flow chart of neural cell experiments. (B) Cell viability assay (n=6). (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fulroscence intensity of Ca 2+ in neuron (n=6). scale bars: 20 µm. (E) Summary data of relative fulroscence intensity of ROS in neuron (n=6). (F-G) Flow cytometry and quantification of cell apoptosis rate (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001.
    Fluo 4 Ca 2+ Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/fluo+4+am/pmc11780535-122-10-15
    Average 90 stars, based on 1 article reviews
    fluo-4 ca 2+ detection kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime ca 2+ detection kit s1061s
    Effects of HLTP on MMP and Ca 2+ ( (A) Flow cytometry to detect cellular MMP, F = 442.9, P < 0.0001; (B) Flow cytometry to detect intracellular Ca 2+ concentration, F = 497.1, P < 0.0001; (C) Fluorescence microscopy to observe intracellular Ca 2+ concentration, Normal ( F = 93.4, P < 0.0001), Fluo-4 ( F = 86.7, P < 0.0001)) (Note: Compared with the Control group: # P < 0.05 and ## P < 0.01; Compared with the Model group: * P < 0.05 and ** P < 0.01; mean ± SD, n = 3; A–C: DFn = 4, DFd = 10; Magnification ×20. Flow cytometry and fluorescence microscopy were used to detect the MMP and Ca 2+ ).
    Ca 2+ Detection Kit S1061s, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/fluo+4+am/pmc11272554-83-3-11
    Average 90 stars, based on 1 article reviews
    ca 2+ detection kit s1061s - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime ca 2+ detection kit s1063s
    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.
    Ca 2+ Detection Kit S1063s, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/calcium+assay+kit/pmc11117216-138-15-21
    Average 90 stars, based on 1 article reviews
    ca 2+ detection kit s1063s - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Nanjing Jiancheng Bioengineering Research Institute Co Ltd ca 2+/mg 2+-atpase detection kit
    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.
    Ca 2+/Mg 2+ Atpase Detection Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/ca2++atpase+assay+kit/pm38677699-79-19-29
    Average 90 stars, based on 1 article reviews
    ca 2+/mg 2+-atpase detection kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Genmed Inc mitochondrial ca 2+ concentration fluorescence detection kit
    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.
    Mitochondrial Ca 2+ Concentration Fluorescence Detection Kit, supplied by Genmed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/mitochondrial+ca2++concentration+quantitative+determination+kit/pmc11016958-42-2-8
    Average 90 stars, based on 1 article reviews
    mitochondrial ca 2+ concentration fluorescence detection kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    GlpBio Technology Inc ros detection kit (2’,7’-dichlorodihydrofluorescein diacetate, dcfh-da, cas: 4091-99-0
    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.
    Ros Detection Kit (2’,7’ Dichlorodihydrofluorescein Diacetate, Dcfh Da, Cas: 4091 99 0, supplied by GlpBio Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/ros+detection+kit++2%E2%80%99+7%E2%80%99+dichlorodihydrofluorescein+diacetate++dcfh+da++cas++4091+99+0/pm38167723-292-4-26
    Average 90 stars, based on 1 article reviews
    ros detection kit (2’,7’-dichlorodihydrofluorescein diacetate, dcfh-da, cas: 4091-99-0 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Transcriptomic results of PRAC-E ameliorating HA. ( A ) Venn diagram of the DEGs from PRAC-E vs. LYSO-6 and LYSO-6 vs. Normal. ( B ) LYSO-6 vs. Normal DEGs volcano plot. ( C ) PRAC-E vs. LYSO-6 DEGs volcano plot. ( D ) Functional enrichment analysis of downregulated DEGs in LYSO-6 vs. Normal. ( E ) Function enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( F ) Pathway enrichment analysis of the downregulated DEGs in LYSO-6 vs. Normal. ( G ) Pathway enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( H ) Ca 2+ -ATPase activity in liver tissue ( n = 6). Data are presented as the mean ± standard deviation (SD) ( n = 6). ** p < 0.01, *** p < 0.001 vs. LYSO-6.

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis

    doi: 10.3390/ijms27062582

    Figure Lengend Snippet: Transcriptomic results of PRAC-E ameliorating HA. ( A ) Venn diagram of the DEGs from PRAC-E vs. LYSO-6 and LYSO-6 vs. Normal. ( B ) LYSO-6 vs. Normal DEGs volcano plot. ( C ) PRAC-E vs. LYSO-6 DEGs volcano plot. ( D ) Functional enrichment analysis of downregulated DEGs in LYSO-6 vs. Normal. ( E ) Function enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( F ) Pathway enrichment analysis of the downregulated DEGs in LYSO-6 vs. Normal. ( G ) Pathway enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( H ) Ca 2+ -ATPase activity in liver tissue ( n = 6). Data are presented as the mean ± standard deviation (SD) ( n = 6). ** p < 0.01, *** p < 0.001 vs. LYSO-6.

    Article Snippet: ALT, AST, ALP, and ultramicro Ca 2+ -ATPase detection kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

    Techniques: Functional Assay, Activity Assay, Standard Deviation

    PRAC-E modulates calcium homeostasis via the cGMP/PKG/ATP2A1 signaling axis, thereby alleviating inflammation and amyloid deposition. ( A , B ) The dose-dependent ( A ) and time-dependent ( B ) effects of LYSO-6 on NCTC1469 cells. ( C ) Screening of the effective concentration of PRAC-E on LYSO-6-stimulated cells. ( D – F ) The levels of IL-6, TNF-α, and IL-8 in the cell culture supernatant. ( G – I ) The levels of SAA, SAP ( G ), and APO-E ( H ) in NCTC1469 cells. ( J ) CR staining of NCTC1469 cells. ( K ) The dose-dependent effects of BAPTA-AM on NCTC1469 cells. ( L , M ) Representative Fluo-4 AM fluorescence staining and semi-quantification of NCTC1469 cells. ( N ) Ca 2+ -ATPase activity in NCTC1469 cells. ( O ) Intracellular cGMP levels in NCTC1469 cells were measured by ELISA. ( P – R ) Western blotting and semi-quantitative analysis of PDE5A, PKG, and ATP2A1 in NCTC1469 cells. Data are presented as the mean ± SD ( n = 6). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. solvent control group; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. LYSO-6; ns, no significance.

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis

    doi: 10.3390/ijms27062582

    Figure Lengend Snippet: PRAC-E modulates calcium homeostasis via the cGMP/PKG/ATP2A1 signaling axis, thereby alleviating inflammation and amyloid deposition. ( A , B ) The dose-dependent ( A ) and time-dependent ( B ) effects of LYSO-6 on NCTC1469 cells. ( C ) Screening of the effective concentration of PRAC-E on LYSO-6-stimulated cells. ( D – F ) The levels of IL-6, TNF-α, and IL-8 in the cell culture supernatant. ( G – I ) The levels of SAA, SAP ( G ), and APO-E ( H ) in NCTC1469 cells. ( J ) CR staining of NCTC1469 cells. ( K ) The dose-dependent effects of BAPTA-AM on NCTC1469 cells. ( L , M ) Representative Fluo-4 AM fluorescence staining and semi-quantification of NCTC1469 cells. ( N ) Ca 2+ -ATPase activity in NCTC1469 cells. ( O ) Intracellular cGMP levels in NCTC1469 cells were measured by ELISA. ( P – R ) Western blotting and semi-quantitative analysis of PDE5A, PKG, and ATP2A1 in NCTC1469 cells. Data are presented as the mean ± SD ( n = 6). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. solvent control group; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. LYSO-6; ns, no significance.

    Article Snippet: ALT, AST, ALP, and ultramicro Ca 2+ -ATPase detection kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

    Techniques: Concentration Assay, Cell Culture, Staining, Fluorescence, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Solvent, Control

    Journal: World Journal of Cardiology

    Article Title: Mechanism of myocardial damage induced by doxorubicin via calumenin-regulated mitochondrial dynamics and the calcium–Cx43 pathway

    doi: 10.4330/wjc.v17.i5.104839

    Figure Lengend Snippet: The concentration of intracellular free Ca 2+ of cardiomyocytes in vivo

    Article Snippet: The intracellular free Ca 2+ in rat cardiac tissues was detected with the intracellular free Ca 2+ detection kit and fluorescence labeling instrument, which were obtained from Shanghai Haohai Biological Technology Co. Ltd. Cardiac tissues were digested to a single-cell suspension, which was treated with reaction reagent and a dye solution.

    Techniques: Concentration Assay

    In vitro effects of the combination hypothermia and MgSO 4 on neurons. (A) The flow chart of neural cell experiments. (B) Cell viability assay (n=6). (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fulroscence intensity of Ca 2+ in neuron (n=6). scale bars: 20 µm. (E) Summary data of relative fulroscence intensity of ROS in neuron (n=6). (F-G) Flow cytometry and quantification of cell apoptosis rate (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Theranostics

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

    doi: 10.7150/thno.104879

    Figure Lengend Snippet: In vitro effects of the combination hypothermia and MgSO 4 on neurons. (A) The flow chart of neural cell experiments. (B) Cell viability assay (n=6). (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fulroscence intensity of Ca 2+ in neuron (n=6). scale bars: 20 µm. (E) Summary data of relative fulroscence intensity of ROS in neuron (n=6). (F-G) Flow cytometry and quantification of cell apoptosis rate (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Techniques: In Vitro, Viability Assay, Fluorescence, Imaging, Flow Cytometry

    In vitro effects of the combination of hypothermia and MgSO 4 on microvessel EC. (A) The flow chart of microvessel EC experiments. (B) Cell viability assay. (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fluorescence intensity of Ca 2+ in ECs. Scale bars: 20 µm. (E) Summary data of relative fluorescence intensity of ROS in ECs. (F-G) Fluorescence imaging and quantification of cellular ZO-1 expression. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Theranostics

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

    doi: 10.7150/thno.104879

    Figure Lengend Snippet: In vitro effects of the combination of hypothermia and MgSO 4 on microvessel EC. (A) The flow chart of microvessel EC experiments. (B) Cell viability assay. (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fluorescence intensity of Ca 2+ in ECs. Scale bars: 20 µm. (E) Summary data of relative fluorescence intensity of ROS in ECs. (F-G) Fluorescence imaging and quantification of cellular ZO-1 expression. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Techniques: In Vitro, Viability Assay, Fluorescence, Imaging, Expressing

    In vitro effects of the combination of hypothermia and MgSO 4 on pericytes. (A) The flow chart of pericyte experiments. (B) Cell viability assay. (C) Quantitative analysis of the area of collagen gel. (D) Quantitative analysis of relative fluorescence intensity of Ca 2+ in pericytes. (E) Fluorescence imaging of intracellular Ca 2+ after reoxygenation 24 h. scale bars: 20 µm. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Theranostics

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

    doi: 10.7150/thno.104879

    Figure Lengend Snippet: In vitro effects of the combination of hypothermia and MgSO 4 on pericytes. (A) The flow chart of pericyte experiments. (B) Cell viability assay. (C) Quantitative analysis of the area of collagen gel. (D) Quantitative analysis of relative fluorescence intensity of Ca 2+ in pericytes. (E) Fluorescence imaging of intracellular Ca 2+ after reoxygenation 24 h. scale bars: 20 µm. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Techniques: In Vitro, Viability Assay, Fluorescence, Imaging

    MgSO 4 as a novel hypothermia infusion solution for promoting AIS recovery by regulating Ca 2+ in NVUs. After I/R injury, the infarction region undergoes severe neural injury, BBB breakdown and microvessel occlusion, which were triggered by Ca 2+ influx into neurons, cerebral microvessel ECs and pericytes. IA-SCSI cooled the brain and promoted partial recovery from neural injury and BBB breakdown but could not rescue microvessel occlusion. In vitro cell experiments confirmed that hypothermia could partially inhibit Ca 2+ influx into neurons and cerebral microvessel ECs but did not affect the total amount of calcium ions in pericytes because hypothermia increased Ca 2+ influx through TRPM8. Moreover, IA-SCMI demonstrated significant recovery from neural injury and BBB breakdown, and enhanced microvessel circulation. The combination of hypothermia and Mg 2+ exerted stronger inhibitory effects on Ca 2+ influx compared with hypothermia alone in NVU cells. MCA, middle cerebral artery; ICA, internal carotid artery; ECA, external carotid artery; CCA, common carotid artery; IA-SCSI, intra-artery selective cooling saline infusion; IA-SCMI, intra-artery selective cooling MgSO 4 infusion; BBB, brain-blood barrier.

    Journal: Theranostics

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

    doi: 10.7150/thno.104879

    Figure Lengend Snippet: MgSO 4 as a novel hypothermia infusion solution for promoting AIS recovery by regulating Ca 2+ in NVUs. After I/R injury, the infarction region undergoes severe neural injury, BBB breakdown and microvessel occlusion, which were triggered by Ca 2+ influx into neurons, cerebral microvessel ECs and pericytes. IA-SCSI cooled the brain and promoted partial recovery from neural injury and BBB breakdown but could not rescue microvessel occlusion. In vitro cell experiments confirmed that hypothermia could partially inhibit Ca 2+ influx into neurons and cerebral microvessel ECs but did not affect the total amount of calcium ions in pericytes because hypothermia increased Ca 2+ influx through TRPM8. Moreover, IA-SCMI demonstrated significant recovery from neural injury and BBB breakdown, and enhanced microvessel circulation. The combination of hypothermia and Mg 2+ exerted stronger inhibitory effects on Ca 2+ influx compared with hypothermia alone in NVU cells. MCA, middle cerebral artery; ICA, internal carotid artery; ECA, external carotid artery; CCA, common carotid artery; IA-SCSI, intra-artery selective cooling saline infusion; IA-SCMI, intra-artery selective cooling MgSO 4 infusion; BBB, brain-blood barrier.

    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Techniques: Infusion Solution, In Vitro, Saline

    Effects of HLTP on MMP and Ca 2+ ( (A) Flow cytometry to detect cellular MMP, F = 442.9, P < 0.0001; (B) Flow cytometry to detect intracellular Ca 2+ concentration, F = 497.1, P < 0.0001; (C) Fluorescence microscopy to observe intracellular Ca 2+ concentration, Normal ( F = 93.4, P < 0.0001), Fluo-4 ( F = 86.7, P < 0.0001)) (Note: Compared with the Control group: # P < 0.05 and ## P < 0.01; Compared with the Model group: * P < 0.05 and ** P < 0.01; mean ± SD, n = 3; A–C: DFn = 4, DFd = 10; Magnification ×20. Flow cytometry and fluorescence microscopy were used to detect the MMP and Ca 2+ ).

    Journal: Frontiers in Pharmacology

    Article Title: Neuroprotective effects of total phenolics from Hemerocallis citrina Baroni leaves through the PI3K/AKT pathway

    doi: 10.3389/fphar.2024.1370619

    Figure Lengend Snippet: Effects of HLTP on MMP and Ca 2+ ( (A) Flow cytometry to detect cellular MMP, F = 442.9, P < 0.0001; (B) Flow cytometry to detect intracellular Ca 2+ concentration, F = 497.1, P < 0.0001; (C) Fluorescence microscopy to observe intracellular Ca 2+ concentration, Normal ( F = 93.4, P < 0.0001), Fluo-4 ( F = 86.7, P < 0.0001)) (Note: Compared with the Control group: # P < 0.05 and ## P < 0.01; Compared with the Model group: * P < 0.05 and ** P < 0.01; mean ± SD, n = 3; A–C: DFn = 4, DFd = 10; Magnification ×20. Flow cytometry and fluorescence microscopy were used to detect the MMP and Ca 2+ ).

    Article Snippet: ROS (S0033S) and Ca 2+ (S1061S) detection kits were obtained from Beyotime.

    Techniques: Flow Cytometry, Concentration Assay, Fluorescence, Microscopy, Control

    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures

    doi: 10.3390/ani14101441

    Figure Lengend Snippet: The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.

    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Techniques: Activity Assay